Nutrient Composition on the Effect of Extracellular Peroxidase Production by Lentinus kauffmanii - under Submerged Culture Condition

An indigenous wood rotting edible Lentinus kauffmanii isolated from Keeriparai forest of Western Ghats was studied for extracellular peroxidase production. Peroxidases are major extracellular lignolytic enzymes involved in lignocellulosic degradation. Physical factors such as pH, temperature and nutritional factors such as carbon, nitrogen sources and amino acids were standardized for the growth and enzyme production of extracellular peroxidase by L. kauffmanii. The optimum pH for both the biomass and the peroxidase production was found to be pH 6.5. Of the different temperature (20C, 25C, 30C, 35C and 40C) tested optimal peroxidase activity (44.595 ± 0.674 UmL) was recorded at 25C. Among the different carbon sources, fructose supported maximum peroxidase activity (53.516 ± 0.952 UmL), while peptone supported the optimal activity (54.454 ± 0.454 UmL) among the different nitrogen sources tested. Of the different amino acids tested, alanine (54.981 ± 0.776 UmL) enhanced the maximum enzyme activity.